Figure 7

DRAM1 regulates cell death through BAX. (a) A549 cells were treated with 3NP (500 μM) for 24, 48 and 72 h, and the cell viability was evaluated. Bars represent mean±S.E.; n=4; **P<0.01; ***P<0.001 versus 0 h. (b) Cell viability was evaluated after transfection of cells with DRAM1 small interfering RNA (siRNA) in the presence or absence of 3NP (500 μM). Bars represent mean±S.E.; n=4; **P<0.01 versus NC; ^##P<0.01 versus NC+3NP. (c) Cell viability was evaluated after transfection of cells with BAX-pcDNA4 or BAX siRNA in the presence or absence of 3NP (500 μM). Bars represent mean±S.E.; n=4; **P<0.01 versus NC; ^#P<0.05 versus NC+3NP; ^$$P<0.01 v ersus NC+3NP. (d) A549 cells were transfected with BID siRNA for 24 h and then treated with 3NP (500 μM) for another 24 h. Bars represent mean±S.E.; n=3; **P<0.01 versus NC; ^#P<0.05 versus 3NP+non-silencing (Non-sil) siRNA. (e) A549 cells were transfected with BAX siRNA or Non-sil siRNA for 24 h and then treated with 3NP (500 μM) for another 24 h. After treatment, apoptotic cells were determined with FACS after Annexin V-FITC and PI staining. NC, Negative control. NC, negative control