Figure 4
From: Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice
Autophagy was induced in mice ovary during neonatal transition. (a) Autophagy induction in the neonatal ovary indicated by immunostaining of LC3 (in green). In the non-suckling groups, mice were separated from the mother immediately after birth. Ovaries were isolated from mice at 0, 3, 6, 12 and 24 h, immediately fixed, cryosectioned, and analyzed by fluorescence microscopy. (b) Quantification of LC3 fluorescent intensity in panel (a). Asterisks indicate statistically significant difference in comparison with the normal group. (c) Survival time of neonatal mice under non-suckling condition. Most of the neonatal mice died during 21–24 h after birth under non-suckling condition. The 24-h survivals were immediately killed for experiments. (d) Morphological analysis of 3 days’ ovaries derived from 20-h non-suckling starvation. (e) Quantification of oocyte and follicle numbers in panel (d). Data are presented as the mean of three experiments±S.E.M. *P<0.05, **P<0.01
