Figure 7
From: Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice
Inhibition of autophagy led to oocytes over-loss through apoptosis under starvation condition. (a) In vitro cultured ovarian tissues were stained by TUNEL. TUNEL signal (in green), nucleus (in red). (b) Percentages of TUNEL-positive oocytes. (c) Morphological analysis of 3 days ovaries derived from in vitro cultured with or without caspase inhibitor. (d) Quantification of the oocyte and follicle numbers in panel (c). Data are presented as the mean of three experiments±S.E.M. *P<0.05, **P<0.01
