Figure 1

PS-1145-mediated inhibition of IKK fails to sensitize thymocytes to TNF killing. (a) Thymocytes from wt or Bad^−/− mice 6-12 weeks of age were put in culture, left untreated or were pretreated with the IKK inhibitor PS-1145 (10 μM) or IKK-VII (10 μM) for 2 h prior TNF (5 ng/ml) simulation. Staurosporine (STS) was used at 100 nM as a control. Viability was assessed over time by Annexin V plus 7-AAD staining and flow cytometry. Bars represent means±S.E.M. of n=6 independent experiments performed in triplicates. (b) Thymocytes from Bad^−/− mice were treated with solvent or IKK inhibitors (10 μM) for 2 h prior TNF stimulation (5 ng/ml) and analyzed by western blot to confirm the activity of IKK inhibitors