Figure 8
From: The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine
Pulmonary inflammation upon instillation of nanoparticles is partially dependent on adenosine production. C57BL/6 mice were instilled with saline solution or nano-TiO2 (5 mg/kg). Lung histology was performed 24 h after instillation to visualise aggregates of nanoparticles and inflammation in tissue (haematoxylin and eosin staining; original magnification, × 1000; micrographs are representatives of 5 mice per group) (a). Simultaneously to the instillation of vehicle or nano-SiO2, mice were treated or not intraperitoneally with adenosine deaminase (ADA, 5 U/mouse). Inflammation parameters were investigated at 6 h. Neutrophil counts (b) KC (c) and MMP-9 (d) contents were measured in the BALF. Myeloperoxidase (MPO) level (e) and IL-1β secretion (f) in lung homogenates were measured. Data are representative of three independent experiments. (*P≤0.05, **P≤0.01, ***P≤0.001)
