Figure 4

H₂O₂ induces concentration and caspase-dependent apoptosis in Eol-1 cells. Eol-1 cells with H₂O₂ (0.3 mM–3.0 mM) (a) or H₂O₂ (1 mM) and Q-VD-OPh (10 μM) (b). Western blotting for cleaved caspase-3 (17/19 kDa) and GAPDH (37 kDa) (c). Data represent mean±S.E.M. n=3. *P<0.05, **P<0.01, ***P<0.001. Western blots representative on three experiments