Figure 5
From: MiR-34c suppresses tumor growth and metastasis in nasopharyngeal carcinoma by targeting MET
MiR-34c affects NPC cell proliferation, migration and invasion by directly targeting MET. (a–f) CNE-2 and SUNE-1 cells were co-transfected with siRNA (siMET or siSCR, 100 nM) and miRNA mimic (miR-34c mimic or miR-Ctrl, 50 nM). Each experiment was independently repeated at least three times. (a) Western blotting analysis of MET protein expression. (b–e) Knockdown of MET markedly inhibited the NPC cell proliferation, migration and invasion, and upregulation of miR-34c did not suppress proliferation, migration and invasion in siMET-transfected CNE-2 and SUNE-1 cells. Representative results of the MTT assay (b), colony formation assay (c), wounding healing assay (d), Transwell migration assay (e) and invasion assay (f). Data are presented as mean±S.D.; **P<0.01, Student’s t-test
