Figure 6
From: MiR-34c suppresses tumor growth and metastasis in nasopharyngeal carcinoma by targeting MET
Overexpression of MET rescued the suppressive effects of miR-34c on NPC cell proliferation, migration and invasion. (a–f) CNE-2 and SUNE-1 cells were co-transfected with miRNA mimic (miR-34c mimic or miR-ctrl, 50 nM) and either the empty pReceiver-M02 vector control (Vector) or pReceiver-M02-MET plasmid- (MET) overexpressing MET (2 μg). (a) Western blot analysis of MET protein expression. Each experiment was independently repeated at least three times. (b–e) Effects of restoration of MET on cell proliferation, migration and invasion. Representative results of the MTT assay (b), colony formation assay (c), wounding healing assay (d), Transwell migration assay (e) and invasion assay (f). Each experiment was independently repeated at least three times. Data are presented as mean±S.D.; **P<0.01, Student’s t-test
