Figure 3
From: TSC2/mTORC1 signaling controls Paneth and goblet cell differentiation in the intestinal epithelium
TSC2 inactivation leads to the increased Notch signaling in intestine. (a) Immunohistochemical (IHC) staining (arrow) for NICD and Hes1 demonstrated increased expression in the intestinal epithelium of TG mice compared with WT mice. (b) Mucosal protein lysates extracted from WT and TG mice were used for western blot detection of NICD and Hes1 protein expression. (c) TSC2-mutant TG mice were treated with or without DBZ for 5 day. The inhibition of Notch signaling by DBZ was demonstrated by staining with NICD (arrow). The goblet cell population, revealed by Alcian blue staining (arrow) and Paneth cells, assessed by IHC staining of lysozyme (arrow), are reduced in the small intestinal epithelium of TSC2-mutant TG mice. Treatment with DBZ restored the goblet and Paneth cell numbers. (Data represent mean±S.D.; *P<0.05 versus WT alone; #P<0.05 versus TG alone). Scale bars, 50 μm
