Figure 4
From: TSC2/mTORC1 signaling controls Paneth and goblet cell differentiation in the intestinal epithelium
TSC2/mTOR signaling pathway controls differentiation of the goblet and Paneth cell lineages. WT- and TSC2-mutant TG mice were treated with or without rapamycin for 6 days. The inhibition of mTORC1 signaling by rapamycin was demonstrated by staining with phospho-S6 (a, arrow). The goblet cell population, revealed by Alcian blue staining and MUC2 immunostaining (b, arrow; c), and Paneth cells, assessed by immunohistochemical staining of lysozyme (d, arrow; e), are reduced in the small intestinal epithelium of TSC2-mutant TG mice. (Data represent mean±S.D.; *P<0.05 versus WT alone; #P<0.05 versus TG alone). Treatment with rapamycin restored the goblet and Paneth cell numbers. Scale bars, 50 μm
