Figure 5
From: TSC2/mTORC1 signaling controls Paneth and goblet cell differentiation in the intestinal epithelium
TSC2/mTOR is an upstream regulator of Notch-Hes1 signaling in the human colon cancer cell line LS174T. (a) LS174T cells were transfected with non-targeting control (NTC) siRNA or siRNA-targeting TSC2. (b) LS174T cells were treated with 100 nM rapamycin for 24 h. (c) LS174T cells were transfected with NTC siRNA or siRNA-targeting mTOR. Total protein was extracted and western blotting performed using anti-NICD, anti-Hes1, anti-TSC2, anti-mTOR, anti-p-S6, anti-S6 and anti-β-actin antibodies. (d) LS174T cells were transfected with NTC siRNA or siRNA-targeting mTOR; total RNA was extracted and MUC2 mRNA levels were determined by real-time RT-PCR. (Data represent mean±S.D.; *P<0.05 versus NTC siRNA)
