Figure 1
From: Targeting MKK3 as a novel anticancer strategy: molecular mechanisms and therapeutical implications
MKK3 depletion is detrimental to cancer but not normal cell proliferation and survival. Efficient MKK3 depletion was achieved in all tested lines (a–d, left panels): Engineered MCF7 (a), HCT116 (b), FB1329 (c) MCF10A (d) -sh/scr and -sh/MKK3 sublines (1.5 × 105 cells/60 mm dish) were challenged with DOX (1.0 μg/ml) and cells collected time dependently (48, 72, 96 and 120 h) to assess the MKK3 depletion efficiency. Then, protein lysates (30 μg /lane) were resolved in SDS–polyacrylamide gel electrophoresis and filter analyzed by western blot analysis with specific anti-MKK3 and anti-β-actin (loading control) antibodies. MKK3 depletion affects cell proliferation and viability of wtp53 tumor but not normal cells (a– d, right panels). Engineered MCF7 (a), HCT116 (b), FB1329 (c) MCF10A (d) -sh/scr and -sh/MKK3 sublines were seeded (2 × 104 cells/6-well plates) in DOX conditions. At the indicated time points, cells were harvested and quantified for viable and death cells by Trypan blue exclusion assays. Results are reported as means and S.D. of three independent experiments. *The significance (P<0.05) of death percent in sh/MKK3 with respect to sh/scr subline
