Figure 2 | Cell Death & Disease

Figure 2

From: Enhanced myeloid differentiation factor 88 promotes tumor metastasis via induction of epithelial–mesenchymal transition in human hepatocellular carcinoma

Figure 2

MyD88 enhances tumor-initiating capacity in HCC. (a) Fold-change in mRNA levels of several stemness-related genes and putative hepatic stem cell markers when MyD88 was overexpressed or knocked down. Fold-change in mRNA expression was calculated by normalization to the control as indicated in Materials and Methods. (b) Flow cytometer analysis of Hep3B cells stained with anti-CD133, anti-EpCAM, and anti-CD90 after MyD88 was knocked down. (c) The SP cell fraction was analyzed in Hep3B cells after MyD88 was knocked down. (d) By Magnetic Bead Cell Sorting, EpCAM+ and EpCAM− populations, CD133+ and CD133− populations were isolated from fresh primary HCC samples, and the mRNA levels of MyD88, EpCAM, and CD133 were tested. (e) Representative images and quantification of the sphere formation in PLC/PRF/5 and Hep3B cells after MyD88 was overexpressed or knocked down, respectively. Scale bar, 100 μm. (f) Tumor incidences in SCID mice injected with the indicated number of shNon- or shMyD88-infected Hep3B cells after 6 weeks are shown. Data represent mean±S.D. * indicates P <0.05

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