Figure 3 | Cell Death & Disease

Figure 3

From: Enhanced myeloid differentiation factor 88 promotes tumor metastasis via induction of epithelial–mesenchymal transition in human hepatocellular carcinoma

Figure 3

MyD88 activates Akt, promoting metastatic potential of HCC cells. (a) MyD88 was overexpressed or knocked down as indicated and the phosphorylation levels of Akt were analyzed by western blot assay. (b, c) The expressions of epithelial marker (E-cadherin) and mesenchymal markers (vimentin and N-cadherin) were detected by western blot assay and immunofluorescence staining after PLC/PRF/5/LV-MyD88 cells were infected with DN-Akt. Scale bar, 25 μm. (d) PLC/PRF/5/LV-MyD88 cells were infected by DN-Akt and the invasive properties were analyzed by invasion assay using Matrigel-coated Corning chamber as described in Materials and Methods. (e) Nude mice were given intrasplenic injections of hepatoma cells and then intragastric administration of MK2206 (the inhibitor of Akt) as described in the Materials and Methods, and photographs of HCC tumors growth in the spleens (above) and livers (below) are shown. The number of tumors in each group (n=4) five weeks after inoculation was counted. Data represent mean±S.D. * indicates P <0.05, ** indicates P <0.01

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