Figure 4

MyD88 associates with p85 to activate PI3-K/Akt signaling. (a) PI3-K was immunoprecipitated and its activity was detected as described in Materials and Methods. (b, c) After overexpression or knockdown of MyD88, the cell lysates were subjected to coimmunoprecipitation and western blot analysis. (d) PLC/PRF/5 cells stably expressing MyD88 were transfected with Δp85, and the phosphorylation level of Akt was analyzed by western blot assay