Figure 1 | Cell Death & Disease

Figure 1

From: The proapoptotic BH3-only proteins Bim and Puma are downstream of endoplasmic reticulum and mitochondrial oxidative stress in pancreatic islets in response to glucotoxicity

Figure 1

ER stress activates intrinsic apoptosis in islet cells. (a–c) Islets from wild-type C57BL/6, Bim−/− or Puma−/− mice were cultured in a control medium or in a medium containing 5 μM thapsigargin for 5 days (a) or 4 days (b), or 10 μg/ml tunicamycin for 4 days (c). The frequency of cells undergoing DNA fragmentation was measured by flow cytometry. Results represent mean±s.e.m. of n=4 independent experiments. **P<0.01 when comparing wild-type to Bim−/− or wild-type to Puma−/− islets cultured with thapsigargin or tunicamycin (two-way ANOVA)

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