Figure 7

Proapoptotic intrinsic pathway genes are upregulated in human islets in type 2 diabetes. Quantitative RT-PCR of human islets isolated from non-diabetic (ND; n=12–14) and age-matched type 2 diabetic (T2D; n=9–10) donors. Relative mRNA expression levels for Bim, Puma, Bax and Bid were calculated by normalizing to the signal for 18S and Actin mRNA in each sample. Results represent mean±s.e.m. *P<0.05, **P<0.01 when comparing gene expression in islets of type 2 diabetic donors with those non-diabetic donors (unpaired t-test)