Figure 6

Astrocyte activation in the hippocampus of Rib-injected mice. Mice treated with saline (a, d, g, and j), Rib at 0.8 g/kg (b, e, h, and k) and at 3.2 g/kg (c, f, i, and l) for 10 days, and the brain slices including hippocampal formation were prepared as described in Materials and Methods and reactive astrocytes were analyzed using an anti-GFAP antibody. Reactive astrocytes were significantly increased in the hippocampal area, and immunohistochemistry shows distribution of the increased reactive astrocytes in the CA1 (d, e and f), CA2 (g, h and i) and CA3 (j, k and l) regions. GFAP immunohistochemistry stained with hematoxylin counterstaining. Quantification results were shown in m. The control value was set as 1.0. All values are expressed as means±S.E.M. **P<0.01