Figure 1 | Cell Death & Disease

Figure 1

From: MicroRNA-23a/b and microRNA-27a/b suppress Apaf-1 protein and alleviate hypoxia-induced neuronal apoptosis

Figure 1

Decreased Apaf-1 gene expression during brain development. (a) Representative western blots of Apaf-1 in cortical samples at different developmental stages (E18, P7, P14, and adult). Each lane was loaded with equal amounts of total protein from the mixed samples of five individuals (n=5, unpaired t-test, *P<0.05, **P<0.01, and ***P<0.001). (b) Semiquantitative RT-PCR detection of Apaf-1 mRNA in the total RNA from cerebral cortices at different developmental stages. Quantitative RT-PCR detection of Apaf-1 mRNA (n=5, unpaired t-test, NS, not significant, **P<0.01). Error bars represent the S.E.M. (c) IHC stain of Apaf-1 in E18 and adult brain tissues. Scale bar represents 250 μm; scale bar (insert) represents 50 μm

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