Figure 7
From: PUMA mediates ER stress-induced apoptosis in portal hypertensive gastropathy
ER stress-activated mitochondrial apoptotic signaling by PUMA induced mucosal apoptosis in PHG. (a) PVL induced significant ER stress signaling in mice. Three mice were used in each group. β-Actin was used as the loading control. (b) Targeted deletion of PUMA significantly repressed ER stress-induced caspase-9 and caspase-3 activation by PVL in mice. (c) Immunohistochemistry staining showed that the expression of GRP78 and cleaved caspase-12 was induced by PVL in mice (brown, × 200). (d) Double immunofluorescence staining of GRP78 and PUMA indicated that PUMA upregulation followed GRP78 induction in the gastric mucosa of PVL mice ( × 200). (e) ER stress signaling pathway was activated in the gastric mucosa of PHG patients. β-Actin was used as the loading control. (f) Double immunofluorescence staining of GRP78 and PUMA also confirmed that PUMA upregulation followed GRP78 induction in the gastric mucosa of human PHG ( × 200)
