Figure 2 | Cell Death & Disease

Figure 2

From: Overcoming inherent resistance to histone deacetylase inhibitors in multiple myeloma cells by targeting pathways integral to the actin cytoskeleton

Figure 2

Genetic signature associated with resistance to HDACi. (a) VENN diagram of genes that are differentially regulated in the sensitive (SENS) versus resistant (RES), intermediate (IM) versus SENS and IM versus RES. Differential expression was defined as probes associated with P-values <0.05, and fold changes >1.5. (b) Unsupervised PCA of sensitive, intermediate and resistant cell lines indicating correlation of gene expression to HDACi responsiveness. Resistant HMCL (red) clustered together, whereas intermediate HMCL (green) had a profile closer to that of the sensitive HMCL (blue). (c) Heat map representation of the 97 genes differentially regulated between the SENS versus RES cell lines. Heat map also includes the expression profile of 97 genes in the intermediate cell lines indicating a GEP overlapping with both sensitive and resistant cell lines. (d) Eight genes were selected for validation of the Illumina HT-12 microarray. Column graphs show relative expression levels of the genes as mean±S.E.M. of sensitive (n=5), intermediate (n=2) and resistant (n=2) cell lines. GraphPad prism was utilized to determine significant differences between each cohort (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001)

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