Figure 2
Transfection of WT mtPAP into RS63-3 and -7 rescued radiosensitivity and DNA repair defects. (a) Transfection of WT mtPAP cDNA (+) into RS63-3 and -7 rescued the radiosensitive (i.e., reduced SF%) phenotype measured by the CSA (at 1 Gy). A (+) denotes transfection with WT mtPAP cDNA and (−) denotes transfection with an empty vector. (b) WT mtPAP (+) or control plasmid (−) was transfected into RS63-3 and -7; the WT mtPAP plasmid rescued the DNA DSB repair defect as measured by the NCA (at 15 Gy). ‘% DNA repair’ represents the return to baseline tail moment (TM) measure of DNA damage 5 h post-IR and is the TMno IR/TM5h. An asterisk denotes statistical significance at P<0.05
