Figure 5
Genes downregulated by both miR-663 and AICD are associated with neurogenesis. (a) Three-way Venn diagram demonstrating the overlap among the miR-663 (P<0.001) and AICD (P<0.05) microarray-downregulated genes. (b) Four-way Venn diagram demonstrating the overlap among the miR-663 (P<0.05), AICD (P<0.05) microarray-downregulated genes and genes with miR-663 target sites predicted by Targetscan. (c) Validation of the gene expression fold change using qPCR. (d) Quantitative flow cytometry analysis of βIII-tubulin expression in differentiated hNSCs transfected with siRNAs and non-targeting control. (e) Overall effects of the downregulation of these genes by siRNA on neuronal differentiation. (f) Dual-luciferase reporter assay shows that miR-663 directly represses the expression luciferase genes bearing 5′-UTR segments of CDK6 and 3′-UTR segments of FBPXL18. Quantification data were analyzed from at least three independent experiments (mean±S.E.M). *P<0.05, **P<0.01, #P<0.001
