Figure 1 | Cell Death & Disease

Figure 1

From: FAS-ligand regulates differential activation-induced cell death of human T-helper 1 and 17 cells in healthy donors and multiple sclerosis patients

Figure 1

Th1 cells are more sensitive to TCR-mediated cell death than other Th profiles. Pools of Th0, Th1, Th17 and Th1/17 clones from the same donor were stimulated with anti-CD3-28 beads, anti-CD3 plate bound and soluble anti-CD28. At 24 h (a and b) and 2-6-24-72 h (c and d) after stimulation, clones were stained for Annexin V and PI and then analysed by flow cytometry. The percentage of Annexin V+/Propidium Iodide (PI)− cells (early apoptotic cells) (c) and Annexin V+ cells (total apoptotic cells) (b and d) is reported in the cumulative graph. A representative experiment (a) and cumulative data of 16 (b) or three (c and d) independent experiments performed on 16 (b) or three (c and d) healthy donors are presented. A paired t-test was used to compare sample condition (defined by different colours) with unstimulated (black). *P<0.05; **P<0.005

Back to article page