Figure 3
From: Loss of Caspase-3 sensitizes colon cancer cells to genotoxic stress via RIP1-dependent necrosis
Induction of necrosis in caspase-3 KO cells by DNA-damaging agents. HCT116 WT and C3KO cells were treated with DMSO or 5-FU (50 μg/ml or as specified). (a) Levels of caspases, RIP1, and HMGB1 in the culture medium in WT and three independent C3KO lines 24 h. (b) Adherent cells stained by crystal violet 48 h after the indicated doses. (c) Fractions of PI+/Annexin V-(non-apoptotic dying) cells at 48 h at the indicated doses. (d) Cellular ATP levels at the indicated times. Values were normalized to the DMSO-treated cells. (e) Fractions of Annexin V+ or PI+/Annexin V- cells 48 h with or without z-VAD (20 μM). (f) Representative images of transmission electron microscopy of cells at 24 h. White arrows denote apoptotic cells, black arrows denote necrotic cells. (c–e) Data are the mean + S.E.M. of triplicate wells. **P<0.01
