Figure 3 | Cell Death & Disease

Figure 3

From: Bid chimeras indicate that most BH3-only proteins can directly activate Bak and Bax, and show no preference for Bak versus Bax

Figure 3

Bid chimeras and BH3 peptides are similar in their specificity for activating both Bak and Bax. (a) Bid chimeras activate Bak to release cytochrome c. Wild-type MLM were treated with the indicated Bid chimeras and supernatant (sup) and pellet (pel) fractions Western blotted for cytochrome c (top panels). Quantification of cytochrome c release allowed estimation of EC50 (bottom panels). (b) Comparison of Bak and Bax activation by Bid chimeras and BH3 peptides. Wild-type MLM (Bak activation) or Bak−/− MLM plus 10 nM Bax (Bax activation) were treated with Bid chimeras or BH3 peptide. Cytochrome c release was quantified as in a. The EC50 values from three or four independent experiments are shown, and in each graph the values from one experiment are represented by the same symbol. Note that EC50 values for BidBad and Bad peptide were not estimated because cytochrome c release was often incomplete (see Figure 4)

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