Figure 1

Ectopic expression of mir-4728 affects cell growth, apoptosis, migration, invasion, and metabolism. (a) Stable cell lines overexpressing mir-4728 precursor or control vector were created from MDA-MB-231 and MDA-MB-453 cells, and cell proliferation was determined by direct cell counting. (b) Stable cell lines with specific miR-4728-3p knockdown or control vector were created from BT474-M1 cells, and cell proliferation was determined by direct cell counting, (c) mir-4728 sensitizes breast cancer cells to Taxol-induced apoptosis, (d) Wound-healing scratch assays and Matrigel-modified Boyden chamber assays, respectively. Representative images are shown for the invasion assay. (e) 3-D colonies grown for 8 days in Matrigel comparing MDA-MB-231 cells stably expressing vector control or mir-4728 overexpression plasmid. (f) Specific knockdown of miR-4728-3p increases cell migration in Boyden chamber assays. All error bars indicate±S.E.M. A P value of <0.05 is indicated by *, P<0.01 by ** and P<0.001 by ***