Figure 4
From: ErbB2-intronic MicroRNA-4728: a novel tumor suppressor and antagonist of oncogenic MAPK signaling
mir-4728 inhibits tumor growth in a xenograft model. (a) Tumor growth in nude mice s.c. injected in the mammary fat pad with MDA-MB-231 cells transfected with control plasmid or mir-4728 expression plasmid (n=9 for control vector group and n=10 for mir-4728 group). (b) Phosphorylated ERK protein levels from total protein lysates from the xenograft tumors and ImageJ quantitation of band intensity. (c) miR-4728-3p expression from xenograft tumors determined by qPCR. (d) miR-4728-3p expression inversely correlated with tumor size as determined by qPCR. Linear regression was performed to fit a line and the equation of the line is Y=−0.8979x+15.62. (e) Tumor growth in nude mice s.c. injected in the mammary fat pad with MDA-MB-231 cells identical to the experiment shown in part a was again repeated and similar results were observed. (f) Specific miR-4728-3p knockdown increases tumor growth. All error bars indicate±S.E.M. A P value of <0.05 is indicated by * and P<0.01 by **
