Figure 2
From: Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53
The DDR regulates Che-1/p53 interaction. (a and b) HCT116 cells were treated with 1 μM Dox (a) or with 20 Gy IR (b). Nuclear extracts were immunoprecipitated with anti-p53 or anti-Che-1 antibodies and analyzed by WB with the indicated abs. (c) GST pull-down assay of HCT116 cells irradiated with 20 Gy performed with GST, GST-Che-1 or GST-p53 Sepharose beads. The bottom panel shows Coomassie blue staining of purified GST proteins. (d) TCEs from HCT116 treated where indicated with 1 μM Dox and calf intestinal alkaline phosphatase (CIAP) were analyzed by WB with the indicated abs. (e) Nuclear extracts from IR-treated HCT116 cells were immunoprecipitated and analyzed by WB with the indicated Abs. (f) HCT116 cells were transiently transfected with pCS2-MT control vector, Myc-Che-1 WT or Myc-Che-1S4A expression vectors and treated with 1 μM Dox. Nuclear extracts were immunoprecipitated with anti-p53 or anti-Myc antibodies and analyzed by WB with the indicated abs
