Figure 2
(a) Schematic construction of Ad genome. WT forward and reverse represented the primer location for identification of wide-type contamination. (b) The proportion of EGFP-positive cells were significantly increased in T24 sphere cells treated with OncoAd.RGD-hTERT-EGFP for 2 days than cells treated with OncoAd.hTERT-EGFP. EGFP-positive cells were detected by fluorescence-activated cell sorting analysis. (c) Comparison on cell viability of T24 sphere cells treated with OncoAd.hTERT-EGFP and OncoAd.RGD-hTERT-EGFP at indicated MOI for 4 days. (d) Examination of 2-day cytotoxicity elicited on T24 sphere cells infected with OncoAd.RGD-hTERT-EGFP and OncoAd.RGD-hTERT-TRAIL. Cell viability was determined by MTT assay. All the experiments were repeated three times and all data shown represented mean±S.D. (n=3). **P<0.01, ***P<0.001
