Figure 3
NSC transplantation influenced the processing of APP and the clearance of Aβ protein in Tg2576 mice brains. (a) At 2.5 months after transplantation, western blot analysis was performed with total lysates from the cortical region and hippocampal region of the brains in each group using 6E10 antibody. (b) Aβ and CT bands were detected and normalized by the amount of APP and GAPDH. In the Tg-sham group mice brain, a lot of Aβ and CT were produced compare with the Tg-NSC group mice. (c) Cortical or hippocampal Aβ level was analyzed by Aβ1-42 ELISA in all groups. The levels of Aβ were highly increased in the cortex and hippocampus of Tg-sham group mice compared with those of age-matched WT mice. Note that the levels of Aβ were decreased by the Tg-NSC group mice. (d) In cortex, the production of neprilysin was increased in 1.6-fold by NSC transplantation compared with the Tg-sham group. (e) Enzymatic activity of the β-secretase from the mice brain lysates was assessed using fluorometric reaction. β-Secretase activity was assessed as time passed. Twenty minutes after adding the substrate, β-secretase activity showed significant difference between WT-sham, WT-NSC, Tg-sham and Tg-NSC group. In Tg-NSC group, BACE activity was decreased compared with the Tg-sham group. *P<0.05, **P<0.01, ***P<0.001 by one-way ANOVA
