Figure 3

YM155 decreases phosphorylation of mTOR in HNSCC cell line. (a) CAL27 cells were treated with indicated doses YM155 for 24 h. Western blot analysis showed the weakened phosphorylation of p-Akt, p-mTOR, p-S6 accompanied with decreased cell cycle markers including p-Rb, cyclin D1 and prosurvival markers Bcl2 and Bcl2l1. (b) Immunofluorescence showed the decreased cytoplasm and membrane expression of p-S6 in YM155 group