Figure 5
Δ122p53 and Δ133p53α stabilize FLp53 but inhibit FLp53 activity. (a) Splenocytes from Δ122p53/mΔpro and mΔpro/- mice were cultured, exposed to 0.2 μg/ml amsacrine, and western blots carried out with an antibody to phosphorylated residue 18 of p53 (Serine 18) and an antibody to the p53 target gene, p21CIP1. Equal loading was determined by Ponceau S staining. (b) Mouse 3T3 cells were transduced with either an empty vector or a retroviral vector expressing Δ122p53. The transduced cells were then exposed to 1 μg/ml of amsacrine and western blotting carried out for p53, Δ122p53 and p21CIP1. (c) A549 cells stably transduced with either an empty vector or Δ133p53α were exposed to 1 ug/ml amsacrine and harvested at the indicated time points and protein levels determined by western blotting. (d) A549 cells were transfected with either non-targeting siRNA or siRNA targeting Δ133p53 for 48 h, treated with 1 μg/ml of amsacrine for 5 h, then protein levels determined by western blotting
