Figure 6
From: LincRNA-p21 acts as a mediator of ING1b-induced apoptosis
Overexpression of human lincRNA-p21 in cells results in apoptosis. (a) Human embryonic kidney cells (HEK293) were transfected with control or lincRNA-p21 plasmids at various concentrations for 24 h, and percentage of cell viability was determined using trypan blue exclusion assays. (b) lincRNA-p21 overexpression causes increased caspase 3 cleavage. Cleaved caspase 3 or α-tubulin protein expression in HEK293 cells transfected with 1 ug/ml of lincRNA-p21 plasmids for 24 h was assessed using western blot analysis. (c) Quantification of lincRNA-p21 expression by qRT-PCR after overexpression of 1 ug/ml of lincRNA-p21 constructs for 24 h in Hep3B cells. (d and e) Cells in c were stained using the TUNEL assay and percentage of TUNEL-positive cells in lincRNA-p21 overexpressed samples were compared with control vector treated cells. RNA levels were normalized to GAPDH expression. Experiments were carried out in triplicates and asterisks indicate P<0.05
