Figure 5 | Cell Death & Disease

Figure 5

From: Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells

Figure 5

Thiostrepton acts synergistically with oxaliplatin to kill CD44+ subpopulations in HCT-15 and HT-29 cells as well as suppress sphere formation of HCT-15 cells. (a) HCT-15 and HT-29 cells were treated with DMSO (Veh), oxaliplatin (Oxa, 25 μM), or thiostrepton (Thio, 5 μM), or both drugs together for 48 h. The number of viable CD44+ (black) and CD44− (white) cells separated by flow cytometry were counted by dye exclusion assays. (b) Spheres formed from HCT-15 cells cultured in defined media containing DMSO (Veh), 15 μM oxaliplatin (Oxa), 5 μM thiostrepton (Thio), or both drugs (Both) for 5 days were stained with MTT and then photographed under a microscope (X40)

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