Figure 1
From: Targeting Mdmx to treat breast cancers with wild-type p53
Elevated Mdmx expression inversely correlated with p53 levels. TMA from luminal, Her 2-positive (Her2+), basal-like BrCas and normal breast were stained for Mdmx (top row), Mdm2 (middle row) and p53 (lower row). Representative samples from the 81 tumor biopsies (for luminal A (Case F8) and luminal B (Case C4), Her2+ and Basal subtypes) and 6 normal breast tissues are shown in a. Tissues were scored according to the proportion of cells stained and the overall intensity in the nucleus, and the findings are presented in b according to BrCa subtypes. Staining intensity ranged from minimum to maximum of 0 to 3, whereas the proportion of cells stained was according to an elevating scale of 0 to 4 (0 to >80%, respectively, as described in the text) and scores were summed to a final possible maximum score of 8. Statistical significance discriminated the Mdmx normal and luminal samples (S.D., P=0.0258) and also normal and Her2 samples (P=0.0055). p53 staining only differed significantly from normal levels in the basal samples (P=0.0216). All the experiments were repeated at least three times. Errors bars represent S.D. of the mean. P-values were calculated by Student’s t-test
