Figure 5
From: Targeting Mdmx to treat breast cancers with wild-type p53
KD of Mdmx promoted cellular senescence in tumor xenografts in vivo. MCF-7 cells expressing Mdmx shRNA or control shRNA (shMdmx wobble control) were injected orthotopically into the mammary fat pads of NSG females (n=6) as described in Figure 4c treatment assay. Following 7 days of DOX treatment, mice were killed and tumors were either cryopreserved in preparation for β-gal staining (senescence marker) or formalin fixed and paraffin embedded for staining with hematoxylin and eosin (H&E; for morphological characterization) or IHC staining for Ki67 (proliferation marker), activated caspase-3 (apoptosis marker) and Mdmx, respectively. Mdmx KD was found to correspond with increased senescence and reduced ki67, even after only 7 days of treatment. The scale bar is 50 μm. Representative tumors from two mice of each group mice are shown
