Figure 1 | Cell Death & Disease

Figure 1

From: Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function

Figure 1

Estrogen receptors differentially regulate autophagy pathway-associated gene expression. (a) Total RNA from SK-01, SK-ERα, SK-ERβ and MCF-7 cells was characterized by using the Human Autophagy Primer Library 1 (HATPL-1) and comparing ER expressing cells to mock-plasmid transfected controls (SK-01). Red numbers indicate an upregulation greater than 1.5 fold, blue numbers indicate an downregulation greater than 1.5 fold, dark red numbers denote a P-value of <0.05, black numbers depict no significant change and n.s. no significant change in gene expression within the whole group. Results represent the mean values of five independent experiments. (b and c) Cartoons display a selection of key autophagic genes of the corresponding PCR array analysis in (a) and show the ‘autophagy-footprint’ of ERα (SK-ERα and MCF-7) and ERβ expressing cells. Genes highlighted in blue were downregulated, in gray not altered in expression whereas genes highlighted in red were upregulated. First numbers in (b) show the regulation in SK-ERα cells and following numbers the regulation in MCF-7 cells. In (c) numbers display the regulation of genes in SK-ERβ cells compares to SK-01. n.s. indicates that there is no significant change in gene expression comparing SK-ERα, SK-ERβ and MCF-7 cells to mock-transfected controls

Back to article page