Figure 2

miR-612 regulated proliferation, migration, and EMT progression of CRC cells. (a) miR-612 was overexpressed in two CRC cell lines, HT29 and HCT116. Real-time PCR was conducted to detect the expression of miR-612. (b) Proliferation activity was conducted by CCK-8 assay at the indicated time after seeding (n=3). (c) Anti-miR-612 was used to inhibit miR-612 in CRC cells. Proliferation activity was conducted by CCK-8 assay at the indicated time after seeding (n=3). (d) miR-612 was overexpressed in two CRC cell lines. The indicated cells were seeded into the upper uncoated inserts with serum-free medium. Cells were allowed to migrate for 12 h at 37 °C. The migrated cells were observed using a microscope and counted. Representative images are shown on the left and summarized results are shown as mean±S.D. from three independent experiments (n=3) on the right. (e) Cells transfected with anti-miR-612 were plated at day 2 after transfection. Medium containing 10% FBS was added to the lower chamber. Cells were allowed to migrate for 12 h at 37 °C. The migrated cells were observed using a microscope and counted. Representative images are shown on the left and summarized results are shown as mean±S.D. from three independent experiments (n=3) on the right. (f–g) The expression of cyclin D1, E-cadherin, and N-cadherin was detected by real-time PCR (f) and western blotting (g) in the indicated CRC cells (n=3). All the data were presented as mean±S.D. from three independent experiments. *P<0.05