Figure 3

miR-612 directly regulated AKT2 expression in CRC cells. (a) The wt, mutated, or deleted AKT2 3′-UTR was transfected into CRC cells with or without synthetic miR-612 mimic. (b) Luciferase activity was determined 36 h after transfection. Data were normalized to the luciferase activity transfected with miR-control (n=3). (c) Expression of AKT2 and p-AKT2 upon miR-612 overexpression or miR-612 inhibition was detected in two CRC cell lines by western blotting. (d) Relationship of relative miR-612 and AKT2 expression. We have drawn the correlation formula: y=−0.1927x+1.505 (x is the expression level of miR-612, y is the expression level of AKT2) (n=20). All the data were presented as mean±S.D. from three independent experiments. *P<0.05