Figure 4

Forced expression of AKT2 rescued impaired CRC growth caused by miR-612. (a) AKT2 expression was detected in NT specimens, and metastatic and non-metastatic CRC specimens by immunohistochemistry. Scale bar, 100 μm. (b) miR-612 expression was measured by western blotting in CRC and NT specimens from five random selected patients with metastatic CRC. (c) miR-612 and AKT2 were ectopically expressed in CRC cells. CCK-8 assay was conducted in the indicated cells (n=3). (d) miR-612 and AKT2 were ectopically expressed in CRC cells. The transwell assay was performed in the indicated cells. Representative images are shown on the top and summarized results are mean±S.D. from three independent experiments (n=3) on the bottom. (e and f) miR-612 and AKT2 were ectopically expressed in CRC cells. Cyclin D1, E-cadherin, and N-cadherin were detected by real-time PCR (e) and western blotting (f) in the indicated CRC cells (n=3). All the data were presented as mean±S.D. from three independent experiments. *^,#P<0.05