Figure 5
From: miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2
miR-612 suppressed tumor growth and metastasis through AKT2 in vivo. (a) CRC cells that stably overexpressed miR-612 or AKT2 were collected, suspended at the density of 1 × 106, and inoculated subcutaneously into the right flank regions of 4-week-old male BALB/c nude mice. Xenograft tumors isolated from nude mice on day 14 in different groups. (b) Size of the tumors formed by subcutaneous injection was measured and calculated every 7 days, completing growth curve of xenograft tumors in different groups (n=6 mice per group). (c) The expression of AKT2 was detected by western blotting in the indicated groups of tumors. (d) CRC cells (2 × 106) that stably expressed miR-612 or AKT2 were produced and injected into the spleen of BALB/c nude mice under anesthesia. Mice were killed 8 weeks after injection, and the livers were surgically excised and subjected to hematoxylin and eosin staining to detect the liver lesion caused by CRC metastasis. Scale bar, 100 μm. All the data were presented as mean±S.D. from three independent experiments. *,#P<0.05
