Figure 2
Chemoresistant NSCLC cells exhibit an activated EMT pathway. (a) Morphological images of chemoresistant A549_R and parental A549 cells. Scale bar, 100 μm. (b) A549_R and A549 cells were immunostained for Vimentin, β-catenin and E-cadherin (green). Nuclei were counterstained with DAPI (blue). Scale bar, 25 μm (c–e) mRNA levels of EMT-TFs and CDH2/N-cadherin in A549_R, H358_R and H460_R cells and the corresponding parental cells (A549, H358 and H460) were analyzed by qPCR. Data are shown as mean±S.D. of three independent experiments (n=3). (f) Different NSCLC cells are at distinct stages along the EMT. mRNA levels of EMT-TFs and Vimentin in PC9, H358, A549 and H460 cells were analyzed by qPCR and results are shown in mean±S.D. of three independent experiments (n=3). (g) NSCLC cells at distinct EMT stages exhibit differential sensitivity to MTA. PC9, H358, A549 and H460 cells treated with the indicated MTA doses for 5 days were analyzed by XTT assay. The results are shown as mean±S.D. of triplicate experiments. *P<0.05; **P<0.01; ***P<0.001
