Figure 4
An activated EMT pathway promotes chemoresistance in primary NSCLC cells and kaempferol enhances chemotherapeutic response to MTA. (a) Micrographic images of the primary NSCLC cells used in this study. Scale bar, 100 μm. (b) The EMT pathway is differentially activated in primary NSCLC cells. The mRNA level of EMT-TFs and Vimentin was analyzed by qPCR. (c) Primary NSCLC cells exhibit differential sensitivity to MTA. The cells were treated for 5 days with various MTA doses and drug response was determined by XTT assay. (d) Kaempferol and SB431542 enhance chemotherapeutic response of primary NSCLC cells to MTA. The primary NSCLC cells treated for 5 days with vehicle, kaempferol (40 μM) or SB431542 (40 μM) were subsequently exposed to MTA for an additional 5 days. Drug response was determined by XTT assay. All the results are shown as mean±S.D. of triplicate experiments
