Figure 1 | Cell Death & Disease

Figure 1

From: ARHI (DIRAS3)-mediated autophagy-associated cell death enhances chemosensitivity to cisplatin in ovarian cancer cell lines and xenografts

Figure 1

Re-expression of ARHI produced cell growth arrest and clonogenic cell death. (a) Re-expression of ARHI inhibited cell growth in short-term cell culture. SKOv3-ARHI cells and Hey-ARHI cells were treated with 1 μg/ml DOX (to induce ARHI (DIRAS3)) for indicated time. Cell viability was measured with SRB analysis. The figure shows the combined values of three independent experiments. The columns indicate the mean, and the bars indicate the S.E. (*P<0.05; **P<0.01 DOX+ versus DOX−). (b) Re-expression of ARHI inhibited clonogenic cell growth. Cells were plated in 6-well plates, at a density of 2000 cells/well and allowed to settle for 24 h. Cells were then treated with 1 μg/ml DOX for 3 days and incubated for up to 14 days. Cell viability was measured by colony counts. Data were obtained from three independent experiments. The columns indicate the mean, and the bars indicate the S.E. (**P<0.01)

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