Figure 3 | Cell Death & Disease

Figure 3

From: ARHI (DIRAS3)-mediated autophagy-associated cell death enhances chemosensitivity to cisplatin in ovarian cancer cell lines and xenografts

Figure 3

ARHI re-expression induced autophagic cell death. (a) ATG5 knockdown blocked ARHI-induced autophagy. SKOv3-ARHI-shControl/shATG5 cells were treated with DOX for 24 h, and then cell lysates were collected and probed with antibodies against LC3, p62, ATG5, ARHI and Actin. (b–f) ATG5 knockdown blocked ARHI-induced cell death. SKOv3-ARHI-shControl/shATG5 and Hey-ARHI-shControl/shATG5 cell viability was measured with SRB assays (b and c) and clonogenic assays (d–f) as described in Figure 1a. Each figure shows the combined values of three independent experiments. The columns indicate the mean, and the bars indicate the S.E. The numbers indicate the ratio of shCtrl DOX− versus shCtrl DOX+ and ratio of shATG5 DOX− versus shATG5 DOX+. Differences of ratio between shCtrl and shATG5 were considered statistically significant at *P<0.05 and **P<0.01

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