Figure 3
From: Hexokinase 2 controls cellular stress response through localization of an RNA-binding protein
Knockdown or inhibition of HK2 restores expression of Bcl-xL and prevents hypertonic stress-induced cell death. (a) HeLa T4+ cells were transfected with HK2-targeting (siHK2) or nontargeting control (siCTRL) siRNAs, or treated with 3-BrPa and subsequently treated with 0.4 M sorbitol. Expression of Bcl-xL, HK2, and actin (loading control) was determined by western blot analysis (left). Bcl-xL expression relative to actin was quantified by densitometry (right). MW marker is indicated on the left. (b) Cells were treated as in (a) and caspase 3 and 7 activity was determined using the CellPlayer Kinetic Caspase-3/7 reagent and the IncuCyte Zoom. The y axis represents the number of fluorescent cells per image normalized to cell confluence; n=3 (3-BrPa treatment) and n=4 (siRNA treatment)
