Figure 3

Analysis of the G2/M checkpoint and DNA damage repair. (a and b) The repair of DNA damage was detected by comet assay. Representative images were on the left. (a) The comet tail moment of 75 cells for each time and condition was quantified by CASP software and normalized to that of no irradiation. (b) The comet tail moment of each time and condition was quantified by CASP software and normalized to that of no irradiation. (b and c) Cells were untreated or irradiated as indicated, then incubated for 1 h at 37 °C before fixation. Mitotic cells were determined by phospho-histone H3 staining and flow cytometry. Each analysis was performed by using 20 000 cells. *P<0.05 and ***P<0.001