Figure 4 | Cell Death & Disease

Figure 4

From: Silencing NFBD1/MDC1 enhances the radiosensitivity of human nasopharyngeal cancer CNE1 cells and results in tumor growth inhibition

Figure 4

NFBD1 regulates γ-H2AX and p-ATM foci formation. (a) IR-induced NFBD1 foci formation. Cells were untreated or irradiated with 8 Gy, fixed and stained with anti-NFBD1 antibodies at the times indicated. (b) and (c) Inhibition of NFBD1 results in defective p-ATM and γ-H2AX foci formation after IR exposure. Cells were irradiated with 8 Gy IR, fixed at 1 post irradiation and stained with the indicated antibodies (b: mouse anti-ATM (pT1981) and rabbit anti-NFBD1; c: rabbit anti-γ-H2AX and mouse anti-NFBD1). (d) CNE1 cells were untreated or irradiated with 8 Gy IR, harvested 1 h later and subjected to western blotting analysis with indicated antibodies. Representative blots were shown with β-actin as loading control

Back to article page