Figure 6

Representative Rad51 and DNA-PKcs foci in cells NC-shRNA or NFBD1-shRNA. Rad51 and DNA-PKcs foci formation were deficient in NFBD1-knockdown cells at early time points after exposure to ionizing radiation. Cells were exposed to 8 Gy and fixed for immunocytochemical analysis of Rad51 and DNA-PKcs at the indicated times. Subnuclear aggregates of Rad51 and DNA-PKcs (foci) were visualized by immunofluorescence staining